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NEWS
2021-08-05
Congratulations to Reza and Matias on the excellent contribution on TE-driven DNA transductions in the human genome
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2021-05-29
"Somatic Functional Deletions of Upstream Open Reading Frame-Associated Initiation and Termination Codons in Human Cancer" was published by MDPI
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2020-12-04
Genome Assembly and Annotation of the California Harvester Ant Pogonomyrmex californicus (Buckley, 1867)
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2020-09-10
"GenoTypeMapper: graphical genotyping on genetic and sequence-based maps" by Deblieck, Fatiukha, Grundmann et al. has been published by Plant Methods.
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2020-03-18
"The multi-comparative 2-n-way genome suite" by Churakov, Zhang, Grundmann et al. has been published by Genome Research.
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2020-03-18
"RAB5A and TRAPPC6B are novel targets for Shiga toxin 2a inactivation in kidney epithelial cells" by Kouzel at al. has been published by Scientific Reports.
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2020-03-05
"Enhancer occlusion transcripts regulate the activity of human enhancer domains via transcriptional interference: a computational perspective" by Pande et al. has been published by Nucleic Acid Research
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2020-01-21
Congratulations Felix Manske on getting the GBM-Masterpreis!
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Name: Jessin Janice J.
Status: Ph.D. student
Email: janicej@uni-muenster.de
Room: 2650.110.205
Phone: +49-251-835300-2

Research interests

Evolutionary fate of Minor spliceosomal introns

Many multicellular eukaryotes have two types of spliceosome for the removal of introns from messenger RNA precursors.  The major (U2) spliceosome processes the vast majority of introns, while the minor (U12) spliceosome removes a small fraction (less than 0.5%) of introns, referred to as U12-type introns.  U12-type introns have distinct sequence elements, but usually occur in genes together with U2-type introns.  A phylogenetic distribution of U12-type introns shows that the minor splicing pathway appeared very early in eukaryotic evolution and has been lost repeatedly. However, over time they tend to be either removed from a gene or convert to U12-type. The conversion occurs either by accumulation of point mutations at the splicing junctions or by activation of U2-type cryptic splicing sites that initially leads to an alternative splicing and is followed by an inactivation of a U12-type splicing signal. With almost two dozens of completely sequenced insect genomes, we can study exact mechanism of these changes. We used comparative genomics approach to track down changes that lead to an intron type switch. In general, loss of U12-type introns is more common than their conversion to U2-type and U12 to U2 conversion occurs more frequently among U12 introns of the GT-AG subtype. Additionally, using phylogenetic approach, we were able to estimate frequency and tempo of these changes.

Role of Minor spliceosomal introns in gene expression and regulation

These U12-type introns having very early origin, are evolving incessantly. Tough they are either deleted or getting converted to U2-type intron, a few of the U12 introns are conserved within the genes, as they play indispensible role (yet unknown) in gene expression. Removal of U12-type intron is relatively much slower than U2-type intron by the spliceosome, thereby, slowing down the gene expression. With the availability of the complete human and mouse genome sequences, we can study the evolution of U12-type intron in the paralogous genes and the impact of the U12-type introns in the gene expression. Moreover, it is interesting to discover the selection pressure of U12-type introns in the gene expression and selection bias of the U12-type introns among the paralogous genes containing U12 introns.

Curriculum

  • 2002-2005: B.Sc. in Botany, specialization in Industrial Microbiology , at Lady Doak College,Madurai, India
    • Project 1: Studies on the extraction and the activity of Catecol oxidase extracted from the green grapes, purple grapes and apple at CLRI, India.
    • Project 2: Studies on the effect of Bacteriocin produced by Lactobacillus sp. on selected Bacterial strains.
  • 2005-2007: M.Sc. in Bioinformatics, at Bharathiyar University, Coimbatore, India.
    • Project: Molecular evolution of Salmonella pathogenecity island insight into the Mechanism of Host specificity at Indian Institute of Science (IISC), India
  • 2007-2008: Junior Research Fellow at the department of MCBL, Indian Institute of Science (IISc), Bangalore.
  • 2008 - now: Ph.D. studies in Bioinformatics at Univeristy of Muenster, Muenster, Germany.

Conferences and workshops

Teaching

Publications
2012-02-29 11:59