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NEWS
2021-08-05
Congratulations to Reza and Matias on the excellent contribution on TE-driven DNA transductions in the human genome
Goto
2021-05-29
"Somatic Functional Deletions of Upstream Open Reading Frame-Associated Initiation and Termination Codons in Human Cancer" was published by MDPI
Goto
2020-12-04
Genome Assembly and Annotation of the California Harvester Ant Pogonomyrmex californicus (Buckley, 1867)
Goto
2020-09-10
"GenoTypeMapper: graphical genotyping on genetic and sequence-based maps" by Deblieck, Fatiukha, Grundmann et al. has been published by Plant Methods.
Goto
2020-03-18
"The multi-comparative 2-n-way genome suite" by Churakov, Zhang, Grundmann et al. has been published by Genome Research.
Goto
2020-03-18
"RAB5A and TRAPPC6B are novel targets for Shiga toxin 2a inactivation in kidney epithelial cells" by Kouzel at al. has been published by Scientific Reports.
Goto
2020-03-05
"Enhancer occlusion transcripts regulate the activity of human enhancer domains via transcriptional interference: a computational perspective" by Pande et al. has been published by Nucleic Acid Research
Goto
2020-01-21
Congratulations Felix Manske on getting the GBM-Masterpreis!
Goto

Anna Neumann

Cancer is one of the most common causes of death world wide and in most cancer patients metastasis is the main cause of death. Osteosarcoma (OS) is the most common primary malignancy of bone, with up to 80% of patients suffering from metastasis or micrometastatic disease at the time of diagnosis. For the metastatic potential of tumours invasiveness plays an important role. The focus of this study is to determine new candidate genes for invasiveness. For that OS cell lines are analysed using a modified Boyden Chamber assay to separate invasive and non-invasive cells. After that total RNA of both fractions is analysed by Illumina hybridisation Arrays (V3 bead arrays). Pair wise comparison (using an S-Plus based evaluation pipeline) yield stable differently expressed genes between invasive and non-invasive cells. These genes are involved in pathways such as cell motility, cell communication or signal transduction. Therefore these candidate genes a) support the established experimental model for metastasis of OS cells and b) give strong clues for a core pattern of metastasis related genes. For functional characterization a combination of knock-down experiments (RNAi) and invasion assay is used. To validate the results RT-PCR and immunohistochemistry on a larger sample using OS-TMAs is processed. Determined genes and pathways are correlated with clinical parameters like metastasis, survival and chemotherapy sensitivity in order to improve the understanding of the biology of OS.

2020-08-05 19:54